Biochemical & cellular assays Abcam - Биохимические и клеточные анализы Abcam

 

 

     Биохимические анализы измеряют и могут использоваться для понимания биохимии и биологии белков, например, для количественного определения метаболитов или измерения активности ферментов. В самом широком смысле биохимические анализы — это все анализы, измеряющие белки и биохимию, включая иммуноанализы.

Клеточные анализы анализируют клеточные процессы, такие как апоптоз или пролиферация клеток. Они также измеряют активность путей внутри клетки, просматривая результат пути, который влияет на поведение клетки (или ее органелл), или маркер, который коррелируется с этим путем. Существует два класса клеточных анализов: 1 - которые требуют генетической модификации самой клетки для получения измеримого результата, и 2 - которые полагаются на химические красители, антитела и белки для получения результатов.

Наборы Abcam для анализов клеточного метаболизма представляют собой простые в использовании оптимизированные наборы, которые можно использовать для более быстрого тестирования связей метаболизма в широком спектре заболеваний и метаболических явлений. Компания Abcam имеет 20-летний опыт производства наборов для анализа метаболизма, поэтому может помочь вам понять модель вашей клетки или заболевания, а также то, как ваша мишень или соединение влияет на интересующий вас метаболический путь/фенотип.

 

Cat. Number Product Assay time General Range Reactive species
ab113851 DCFDA / H2DCFDA - Cellular ROS Assay Kit 40m    
ab109721 Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) 3h 30m   Mouse, Rat, Cow, Human
ab109902 Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit 3h 30m   Mouse, Rat, Cow, Human
ab14085 Annexin V-FITC Apoptosis Staining / Detection Kit 10m    
ab66110 TUNEL Assay Kit - BrdU-Red 3h   Mammals
ab65311 Cytochrome c Release Assay Kit 3h   Mouse, Rat, Human
ab156065 SIRT1 Activity Assay Kit (Fluorometric) 1h   Mouse, Rat, Human
ab113852 TMRE-Mitochondrial Membrane Potential Assay Kit 35m    
ab113849 Luminescent ATP Detection Assay Kit 30m    
ab290021 Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody [5F51BB5AG7]     Human, Caenorhabditis elegans, Mouse, Cow, Rat
ab113467 DNMT Activity Assay Kit (Colorimetric) 3h 45m   Mouse, Rat, Human, Drosophila melanogaster, Plants
ab115348 Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit 4h 60 - 250 µg/mL Mouse, Rat, Cow, Dog, Human
ab109908 Complex II Enzyme Activity Microplate Assay Kit 3h   Mouse, Rat, Cow, Human
ab176749 Apoptosis/ Necrosis Assay Kit (blue, green, red) 1h    
ab109911 Complex IV Rodent Enzyme Activity Microplate Assay Kit 6h   Mouse, Rat
ab252887 Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric)     Mouse, Human
ab133112 NFkB p65 Transcription Factor Assay Kit 3h 30m   Mouse, Rat, Human
ab150686 Trichrome Stain Kit (Connective Tissue Stain)      
ab245880 H&E Staining Kit (Hematoxylin and Eosin)      
ab118970 Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) 1h 20m    
ab206386 TUNEL Assay Kit - HRP-DAB 5h    
ab133406 ECL Substrate Kit (High Sensitivity)      
ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) 1h    
ab65390 Cholesterol Assay Kit - HDL and LDL/VLDL 1h    
ab83366 Iron Assay Kit (Colorimetric) 1h 30m 8 - 400 µM  
ab119692 Citrate Synthase Activity Assay Kit 3h 15m 1.56 - 100 µg/mL Cow, Human
ab219801 EdU Assay / EdU Staining Proliferation Kit (iFluor 488)      
ab184867 NQO1 Activity Assay Kit 30m   Mouse, Rat, Cow, Human
ab119693 Malate Dehydrogenase 2 (MDH2) Activity Assay   0.78 - 200 µg/mL Mouse, Rat, Human
ab102505 Calcium Assay Kit (Colorimetric) 20m 0.2 - 1 mM Mammals
ab243377 Lipid Peroxidation Assay Kit (Cell-based)      
ab109909 Complex IV Human Enzyme Activity Microplate Assay Kit     Cow, Human, Pig
ab83355 ATP Assay Kit (Colorimetric/Fluorometric) 1h    
ab211091 MTT Assay Kit (Cell Proliferation) 3h 15m   Mammals
ab139418 Propidium Iodide Flow Cytometry Kit      
ab65336 Triglyceride Assay Kit - Quantification 1h 20m    
ab228554 Cell Counting Kit 8 (WST-8 / CCK8)      
ab235697 Cell Invasion Assay (Basement Membrane), 96-well, 8 µm     Mouse, Human
ab236206 DHE (Dihydroethidium) Assay Kit - Reactive Oxygen Species     Human
ab185910 Histone H3 Modification Multiplex Assay Kit (Colorimetric) 2h 30m 20 - 500 ng/well Mouse, Rat, Human
ab65354 Superoxide Dismutase Activity Assay Kit (Colorimetric) 30m   Mammals
ab109714 ATP synthase Enzyme Activity Microplate Assay Kit 6h   Rat, Cow, Human
ab139484 Autophagy Assay Kit 30m    
ab136955 Glucose Uptake Assay Kit (Colorimetric) 3h   Mammals
ab150674 Iron Stain Kit (Prussian Blue Stain)      
ab150667 Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain)      
ab115091 Histone H3 Total Quantification Kit (Colorimetric) 2h 30m 20 - 1000 ng/well Mouse, Rat, Human
ab65331 L-Lactate Assay Kit (Colorimetric) 30m 0.02 - 10 mM  
ab138881 GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) 30m    
ab126584 HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit     Human
ab126700 HIF1a + PDK1 Hypoxia Response Human Flow Cytometry Kit     Human
ab65330 L-Lactate Assay Kit (Colorimetric/Fluorometric) 40m 0.001 - 10 mM Mammals
ab207223 Nrf2 Transcription Factor Assay Kit (Colorimetric) 3h 30m   Mouse, Rat, Human
ab138880 cAMP Assay Kit (Competitive ELISA, Fluorometric) 3h    
ab176736 Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter     Mammals
ab65340 Creatinine Assay Kit 1h    
ab109712 Aconitase Activity Assay Kit 2h    
ab105134 Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) 1h 20m   Mammals
ab65302 Cathepsin D Activity Assay Kit (Fluorometric) 2h   Mammals
ab228551 Hoechst 33342 Staining Dye Solution      
ab113850 JC-1 - Mitochondrial Membrane Potential Assay Kit 1h    
ab112146 MMP Activity Assay Kit (Fluorometric - Green) 60m    
ab150680 Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) 45m    
ab139476 ROS/Superoxide Detection Assay Kit (Cell-based) 90m    
ab238544 Comet Assay Kit (3-well slides)      
ab102530 Glutathione Peroxidase Assay Kit (Colorimetric) 40m   Mammals
ab150675 Luxol Fast Blue Stain Kit (Myelin Stain)      
ab65329 Total Antioxidant Capacity Assay Kit 2h    
ab83362 Urea Assay Kit 1h 0.5 - 5 nmol/well  
ab83464 Catalase Activity Assay Kit (Colorimetric/Fluorometric) 40m   Mammals
ab65359 Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation 1h    
ab110417 Cytochrome c Apoptosis ICC Antibody Kit     Mouse, Rat, Human
ab222421 EdU Assay / EdU Staining Proliferation Kit (iFluor 647)      
ab105136 Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) 1h   Mammals
ab65328 Nitric Oxide Assay Kit (Colorimetric) 1h 30m 1 - 10 nmol/well Mammals
ab156066 SIRT2 Activity Assay Kit (Fluorometric)     Mouse, Rat, Human
ab150687 Von Kossa Stain Kit (Calcium Stain)      
ab139482 ER Staining Kit - Red Fluorescence - Cytopainter     Mammals
ab136957 Hexokinase Activity Assay Kit (Colorimetric) 1h   Mammals
ab222941 Hydroxyproline Assay Kit (Colorimetric) 3h 0.1 - 1 µg Mammals
ab185912 m6A RNA Methylation Assay Kit (Colorimetric) 3h 45m 10 - 500 ng/mL  
ab133101 PPAR gamma Transcription Factor Assay Kit     Mouse, Rat, Human
ab211094 Adenosine Assay Kit (Fluorometric) 15m   Mammals
ab83369 Alkaline Phosphatase Assay Kit (Colorimetric) 1h 10m 10 - 250 µU Mammals
ab155901 Creatine Kinase Activity Assay Kit (Colorimetric) 1h 2 - 10 nmol/well Mammals
ab235979 NETosis Assay Kit   0.56 - 36 mU/well Human
ab107921 Proteasome Activity Assay Kit 1h   Mammals
ab65342 Pyruvate Assay Kit 40m 1 - 200 µM  
ab102523 Amylase Assay Kit (Colorimetric) 40m   Mammals
ab133116 Cholesterol Assay Kit (Cell-Based) 1h 15m   Mammals
ab228549 DAPI Staining Solution      
ab65341 Free Fatty Acid Assay Kit - Quantification 60m    
ab204732 Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay Kit (Colorimetric) 20m   Mammals
ab169558 Glycogen Assay Kit II (Colorimetric) 1h 4 - 40 µg/mL Mammals
ab287858 Live/Dead Cell Viability Assay Kit     Mammals
ab245884 Movat Pentachrome Stain Kit (Modified Russell-Movat)      
ab204718 Succinate Assay Kit (Colorimetric) 30m   Mammals
ab66108 TUNEL Assay Kit - FITC 2h 30m    
ab138871 Acetylcholinesterase Assay Kit (Colorimetric) 30m    
ab83360 Ammonia Assay Kit 1h    
ab65308 Calpain Activity Assay Kit 2h   Mammals
ab287852 Cell Cycle Analysis Kit     Mammals
ab150669 Fontana-Masson Stain Kit (Melanin Stain)      
ab112134 JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) 1h    
ab102526 LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) 30m 1 - 100 mU/mL  
ab65349 NADP/NADPH Assay Kit 2h    
ab65351 Senescence Detection Kit 1h 10m    
ab204726 Angiogenesis Assay Kit (In Vitro) 6h    
ab105135 Aspartate Aminotransferase Activity Assay Kit 1h    
ab125306 BrdU Immunohistochemistry Kit      
ab176735 Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter     Mammals
ab238535 DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates)      
ab197011 Glutamine Assay Kit (Colorimetric) 90m   Mammals
ab233471 Lipid Peroxidation (MDA) Assay Kit (Colorimetric)      
ab185433 Lipolysis Assay Kit (Colorimetric) 40m 2 - 10 nmol/well Mammals
ab150678 Oil Red O Stain Kit (Lipid Stain)      
ab133107 PPAR alpha Transcription Factor Assay Kit     Mouse, Rat, Human
ab156067 SIRT3 Activity Assay Kit (Fluorometric) 40m   Human
ab150662 Alcian Blue Stain Kit (pH 2.5, Mucin Stain)      
ab155893 ALDH Activity Assay Kit (Colorimetric) 30m   Mammals
ab102536 BCA Protein Assay Kit 1h 30m 0.5 - 30 µg  
ab39401 Caspase-3 Assay Kit (Colorimetric) 2h    
ab235696 Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm)     Mouse, Human
ab65345 Choline/Acetylcholine Assay Kit 40m 0.01 - 5 nmol/well Mammals
ab232855 Crystal violet Assay Kit (Cell viability) 1h   Mammals
ab211070 D 2 Hydroxyglutarate Assay Kit (Colorimetric)     Mammals
ab65333 Glucose Assay Kit 40m 1 - 10000 µM  
ab83389 Glutamate Assay Kit 40m    
ab197010 MTS Assay Kit (Cell Proliferation) (Colorimetric) 4h    
ab65348 NAD/NADH Assay Kit (Colorimetric) 2h 400 - 2000 nM  
ab178020 Protein Carbonyl Assay Kit (Western Blot)      
ab315370 Spinal Astrocytes  - Human iPSC derived cells (Male, WC-30)      
ab222942 Total Collagen Assay Kit (Perchlorate-Free) 3h 0.5 - 10 µg Mammals
ab204695 Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric)   10 - 100 µU Mammals
ab39383 Caspase-3 Assay Kit (Fluorometric) 2h    
ab234056 DNase I Assay Kit (Fluorometric)     Mammals
ab133104 HIF-1 alpha Transcription Factor Assay Kit     Mouse, Rat, Human
ab156064 Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) 1h   Mammals
ab126287 Protein Carbonyl Content Assay Kit 1h 30m    
ab155891 Total Carbohydrate Assay Kit - Quantification 30m    

 

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